Composite

Part:BBa_K4805011:Design

Designed by: Tianyin Zhao   Group: iGEM23_LINKS-China   (2023-10-12)


pTEF2-ERG20_F96W-tSSA1-pPGK1-ClSS_ S532A,F441V-tPGK1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1302
    Illegal BglII site found at 1363
    Illegal BglII site found at 2828
    Illegal BglII site found at 3917
    Illegal XhoI site found at 1479
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1018
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1465
    Illegal BsaI site found at 1726
    Illegal BsaI.rc site found at 1715
    Illegal BsaI.rc site found at 2838


Design Notes

composite


Source

composite

References